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pcdna3 ha arf6q67l  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 ha arf6q67l
    Pcdna3 Ha Arf6q67l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+ha+arf6q67l/pcDNA3+HA+Arf6+ActQ67L+(Plasmid+%2310835)/pm34423453-257-17-18
    Average 92 stars, based on 8 article reviews
    pcdna3 ha arf6q67l - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Dominant Negative Mutation:

    Article Title: A Brucella effector modulates the Arf6-Rab8a GTPase cascade to promote intravacuolar replication.
    Article Snippet: Dominant negative human Rab8aT22N was digested from pEGFP-C1-Rab8aT22N (Hattula & Per€anen, 2000) using EcoRI and BamHI restriction sites and ligated into pmCherry-C1 (Clontech) to generate pmCherry-Rab8aT22N. .. Wild-type, constitutively active, and dominant negative human Arf6, Arf6Q67L, and Arf6T27N were amplified from pcDNA3-HA-Arf6 (Addgene #10834), pcDNA3-HA-Arf6Q67L (Addgene #10835), or pcDNA3-HA-Arf6T27N (Addgene #10831) using primers WSU0433 andWSU0437 and cloned a 2021 The Authors The EMBO Journal 40: e107664 | 2021 17 of 23 D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2406:7400:45:ff34:a957:6385:d96d:260. into either pEGFP-N1 or pmCherry-N1 (Clontech) using EcoRI and KpnI restriction sites to generate pmEGFP-N1-Arf6, pEGFPN1-Arf6Q67L, pEGFP-N1-Arf6T27Nor pmCherry-N1-Arf6, pmCherryN1-Arf6Q67L, and pmCherry-N1-Arf6T27N. .. Human ACAP1 cDNA was originally amplified from pCMV6-AC-GFP-ACAP1 (OriGene #RG203724) using primers RC451 and RC452 and cloned into pCMV-myc (Clontech) using the EcoRI restriction site to generate pCMV-myc-ACAP1.

    Amplification:

    Article Title: A Brucella effector modulates the Arf6-Rab8a GTPase cascade to promote intravacuolar replication.
    Article Snippet: Dominant negative human Rab8aT22N was digested from pEGFP-C1-Rab8aT22N (Hattula & Per€anen, 2000) using EcoRI and BamHI restriction sites and ligated into pmCherry-C1 (Clontech) to generate pmCherry-Rab8aT22N. .. Wild-type, constitutively active, and dominant negative human Arf6, Arf6Q67L, and Arf6T27N were amplified from pcDNA3-HA-Arf6 (Addgene #10834), pcDNA3-HA-Arf6Q67L (Addgene #10835), or pcDNA3-HA-Arf6T27N (Addgene #10831) using primers WSU0433 andWSU0437 and cloned a 2021 The Authors The EMBO Journal 40: e107664 | 2021 17 of 23 D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2406:7400:45:ff34:a957:6385:d96d:260. into either pEGFP-N1 or pmCherry-N1 (Clontech) using EcoRI and KpnI restriction sites to generate pmEGFP-N1-Arf6, pEGFPN1-Arf6Q67L, pEGFP-N1-Arf6T27Nor pmCherry-N1-Arf6, pmCherryN1-Arf6Q67L, and pmCherry-N1-Arf6T27N. .. Human ACAP1 cDNA was originally amplified from pCMV6-AC-GFP-ACAP1 (OriGene #RG203724) using primers RC451 and RC452 and cloned into pCMV-myc (Clontech) using the EcoRI restriction site to generate pCMV-myc-ACAP1.

    Clone Assay:

    Article Title: A Brucella effector modulates the Arf6-Rab8a GTPase cascade to promote intravacuolar replication.
    Article Snippet: Dominant negative human Rab8aT22N was digested from pEGFP-C1-Rab8aT22N (Hattula & Per€anen, 2000) using EcoRI and BamHI restriction sites and ligated into pmCherry-C1 (Clontech) to generate pmCherry-Rab8aT22N. .. Wild-type, constitutively active, and dominant negative human Arf6, Arf6Q67L, and Arf6T27N were amplified from pcDNA3-HA-Arf6 (Addgene #10834), pcDNA3-HA-Arf6Q67L (Addgene #10835), or pcDNA3-HA-Arf6T27N (Addgene #10831) using primers WSU0433 andWSU0437 and cloned a 2021 The Authors The EMBO Journal 40: e107664 | 2021 17 of 23 D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2406:7400:45:ff34:a957:6385:d96d:260. into either pEGFP-N1 or pmCherry-N1 (Clontech) using EcoRI and KpnI restriction sites to generate pmEGFP-N1-Arf6, pEGFPN1-Arf6Q67L, pEGFP-N1-Arf6T27Nor pmCherry-N1-Arf6, pmCherryN1-Arf6Q67L, and pmCherry-N1-Arf6T27N. .. Human ACAP1 cDNA was originally amplified from pCMV6-AC-GFP-ACAP1 (OriGene #RG203724) using primers RC451 and RC452 and cloned into pCMV-myc (Clontech) using the EcoRI restriction site to generate pCMV-myc-ACAP1.



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    pcdna3 ha arf6q67l  (Addgene inc)
    92
    Addgene inc pcdna3 ha arf6q67l
    Pcdna3 Ha Arf6q67l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+ha+arf6q67l/pcDNA3+HA+Arf6+ActQ67L+(Plasmid+%2310835)/pm34423453-257-17-18
    Average 92 stars, based on 1 article reviews
    pcdna3 ha arf6q67l - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

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